Notch1 promotes the pericyte-myofibroblast transition in idiopathic pulmonary fibrosis by the PDGFR/ROCK1 sign pathway
The targets of this research have been to analyze the position of the Notch1/PDGFRβ/ROCK1 signaling pathway within the pathogenesis of pulmonary fibrosis and to discover the opportunity of treating fibrosis by focusing on Notch1. Lung tissues from sufferers with pulmonary fibrosis have been examined for the expression of Notch1/PDGFRβ/ROCK1 utilizing RT-qPCR, western blotting, and immunostaining.
Cultured mouse lung pericytes have been transfected with Notch1-overexpressed vectors or shRNA focusing on PDGFRβ/ROCK1 to look at cell behaviors, together with proliferation, cell cycle arrest, and differentiation towards myofibroblasts.
Lastly, a mouse pulmonary fibrosis mannequin was ready, and a Notch1 inhibitor was administered to watch tissue morphology and pericyte cell behaviors. Human pulmonary fibrotic tissues offered with overexpression of Notch1, PDGFRβ, and ROCK1, along with a outstanding transition of pericytes into myofibroblasts.
In cultured mouse lung pericytes, overexpression of Notch1 led to the accelerated proliferation and differentiation of cells, and it additionally elevated the expression of the PDGFRβ and ROCK1 proteins. The knockdown of PDGFRβ/ROCK1 in pericytes remarkably suppressed pericyte proliferation and differentiation.
As additional substantiation, the administration of a Notch1 inhibitor in a mouse mannequin of lung fibrosis inhibited the PDGFRβ/ROCK1 pathway, suppressed pericyte proliferation and differentiation, and alleviated the severity of fibrosis.
Our outcomes confirmed that the Notch1 signaling pathway was aberrantly activated in pulmonary fibrosis, and this pathway could facilitate illness development by way of mediating pericyte proliferation and differentiation. The inhibition of the Notch1 pathway could present one promising remedy technique for pulmonary fibrosis.
Titin (TTN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB667Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Titin (TTN). This antibody is labeled with APC-Cy7. |
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Melanotransferrin (MFI2) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB677Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Melanotransferrin (MFI2). This antibody is labeled with APC-Cy7. |
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Cholesterol (CH) Polyclonal Antibody (General), APC-Cy7 |
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| 4-PAB701Ge01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Cholesterol (CH). This antibody is labeled with APC-Cy7. |
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Glycocalicin (GC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB710Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycocalicin (GC). This antibody is labeled with APC-Cy7. |
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Fascin (FSCN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB757Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Fascin (FSCN). This antibody is labeled with APC-Cy7. |
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Fascin (FSCN) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB757Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Fascin (FSCN). This antibody is labeled with APC-Cy7. |
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Neprilysin (NEP) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB785Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Neprilysin (NEP). This antibody is labeled with APC-Cy7. |
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Neprilysin (NEP) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB785Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with APC-Cy7. |
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Neprilysin (NEP) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB785Ra02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with APC-Cy7. |
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Arginine (Arg) Polyclonal Antibody (General), APC-Cy7 |
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| 4-PAB938Ge01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Arginine (Arg). This antibody is labeled with APC-Cy7. |
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Epiregulin (EREG) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB945Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Epiregulin (EREG). This antibody is labeled with APC-Cy7. |
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Acrosin (ACR) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB947Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Acrosin (ACR). This antibody is labeled with APC-Cy7. |
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Hepcidin (Hepc) Polyclonal Antibody (Bovine), APC-Cy7 |
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| 4-PAB979Bo01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Hepcidin (Hepc). This antibody is labeled with APC-Cy7. |
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Hepcidin (Hepc) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB979Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hepcidin (Hepc). This antibody is labeled with APC-Cy7. |
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Hepcidin (Hepc) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB979Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Hepcidin (Hepc). This antibody is labeled with APC-Cy7. |
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Hepcidin (Hepc) Polyclonal Antibody (Pig), APC-Cy7 |
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| 4-PAB979Po01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Hepcidin (Hepc). This antibody is labeled with APC-Cy7. |
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Hepcidin (Hepc) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB979Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Hepcidin (Hepc). This antibody is labeled with APC-Cy7. |
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Hemopexin (HPX) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB986Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with APC-Cy7. |
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Hemopexin (HPX) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB986Hu02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with APC-Cy7. |
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Hemopexin (HPX) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB986Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with APC-Cy7. |
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Hemopexin (HPX) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB986Ra02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with APC-Cy7. |
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Hemojuvelin (HJV) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB995Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hemojuvelin (HJV). This antibody is labeled with APC-Cy7. |
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Lipopolysaccharide (LPS) Polyclonal Antibody (General), APC-Cy7 |
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| 4-PAB526Ge01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Lipopolysaccharide (LPS). This antibody is labeled with APC-Cy7. |
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Palladin (PALLD) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB590Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Palladin (PALLD). This antibody is labeled with APC-Cy7. |
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Neuraminidase (NEU) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB611Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Neuraminidase (NEU). This antibody is labeled with APC-Cy7. |
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Myostatin (MSTN) Polyclonal Antibody (Bovine), APC-Cy7 |
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| 4-PAB653Bo01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Myostatin (MSTN). This antibody is labeled with APC-Cy7. |
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Myostatin (MSTN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB653Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with APC-Cy7. |
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Myostatin (MSTN) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB653Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Myostatin (MSTN). This antibody is labeled with APC-Cy7. |
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Myostatin (MSTN) Polyclonal Antibody (Pig), APC-Cy7 |
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| 4-PAB653Po01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Myostatin (MSTN). This antibody is labeled with APC-Cy7. |
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Myostatin (MSTN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB653Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with APC-Cy7. |
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Prekallikrein (PK) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB801Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Prekallikrein (PK). This antibody is labeled with APC-Cy7. |
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Versican (VS) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB817Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Versican (VS). This antibody is labeled with APC-Cy7. |
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Versican (VS) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB817Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Versican (VS). This antibody is labeled with APC-Cy7. |
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Vinculin (VCL) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB839Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with APC-Cy7. |
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Paxillin (Pax) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB844Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Paxillin (Pax). This antibody is labeled with APC-Cy7. |
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Nitrotyrosine (NT) Polyclonal Antibody (General), APC-Cy7 |
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| 4-PAB863Ge01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Nitrotyrosine (NT). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Bovine), APC-Cy7 |
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| 4-PAB908Bo01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB908Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB908Hu02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB908Hu03-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB908Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB908Mu02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB908Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Aggrecan (AGC) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB908Ra02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Aggrecan (AGC). This antibody is labeled with APC-Cy7. |
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Hemoglobin (HB) Polyclonal Antibody (Bovine), APC-Cy7 |
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| 4-PAB409Bo01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Hemoglobin (HB). This antibody is labeled with APC-Cy7. |
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Hemoglobin (HB) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB409Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Hemoglobin (HB). This antibody is labeled with APC-Cy7. |
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Hemoglobin (HB) Polyclonal Antibody (Pig), APC-Cy7 |
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| 4-PAB409Po01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Hemoglobin (HB). This antibody is labeled with APC-Cy7. |
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Hemoglobin (HB) Polyclonal Antibody (Rabbit), APC-Cy7 |
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| 4-PAB409Rb51-APC-Cy7 | Cloud-Clone |
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Description: A Guinea polyclonal antibody against Rabbit Hemoglobin (HB). This antibody is labeled with APC-Cy7. |
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Acetylcholinesterase (AchE) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB447Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Acetylcholinesterase (AchE). This antibody is labeled with APC-Cy7. |
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Acetylcholinesterase (AchE) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB447Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Acetylcholinesterase (AchE). This antibody is labeled with APC-Cy7. |
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Acetylcholinesterase (AchE) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB447Mu02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Acetylcholinesterase (AchE). This antibody is labeled with APC-Cy7. |
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Acetylcholinesterase (AchE) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB447Ra02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Acetylcholinesterase (AchE). This antibody is labeled with APC-Cy7. |
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Ovalbumin (OVA) Polyclonal Antibody (Chicken), APC-Cy7 |
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| 4-PAB459Ge01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Ovalbumin (OVA). This antibody is labeled with APC-Cy7. |
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Fibromodulin (FMOD) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB494Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Fibromodulin (FMOD). This antibody is labeled with APC-Cy7. |
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Fibromodulin (FMOD) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB494Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Fibromodulin (FMOD). This antibody is labeled with APC-Cy7. |
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Lumican (LUM) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB496Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Lumican (LUM). This antibody is labeled with APC-Cy7. |
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Lumican (LUM) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB496Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Lumican (LUM). This antibody is labeled with APC-Cy7. |
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Dystrophin (DMD) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB503Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Dystrophin (DMD). This antibody is labeled with APC-Cy7. |
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Dystrophin (DMD) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB503Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Dystrophin (DMD). This antibody is labeled with APC-Cy7. |
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Caldesmon (CALD) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB324Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Caldesmon (CALD). This antibody is labeled with APC-Cy7. |
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Involucrin (iNV) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB331Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Involucrin (iNV). This antibody is labeled with APC-Cy7. |
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Involucrin (iNV) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB331Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Involucrin (iNV). This antibody is labeled with APC-Cy7. |
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Involucrin (iNV) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB331Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Involucrin (iNV). This antibody is labeled with APC-Cy7. |
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Involucrin (iNV) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB331Ra02-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Involucrin (iNV). This antibody is labeled with APC-Cy7. |
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Protectin (CD59) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB336Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Protectin (CD59). This antibody is labeled with APC-Cy7. |
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Protectin (CD59) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB336Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Protectin (CD59). This antibody is labeled with APC-Cy7. |
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Protectin (CD59) Polyclonal Antibody (Pig), APC-Cy7 |
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| 4-PAB336Po01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Protectin (CD59). This antibody is labeled with APC-Cy7. |
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Protectin (CD59) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB336Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Protectin (CD59). This antibody is labeled with APC-Cy7. |
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Protectin (CD59) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB336Si01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Protectin (CD59). This antibody is labeled with APC-Cy7. |
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Elastin (ELN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB337Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Elastin (ELN). This antibody is labeled with APC-Cy7. |
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Elastin (ELN) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB337Hu08-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Elastin (ELN). This antibody is labeled with APC-Cy7. |
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Elastin (ELN) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB337Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Elastin (ELN). This antibody is labeled with APC-Cy7. |
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Prolylcarboxypeptidase (PRCP) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB253Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Prolylcarboxypeptidase (PRCP). This antibody is labeled with APC-Cy7. |
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Prolylcarboxypeptidase (PRCP) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB253Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Prolylcarboxypeptidase (PRCP). This antibody is labeled with APC-Cy7. |
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Prolylcarboxypeptidase (PRCP) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB253Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Prolylcarboxypeptidase (PRCP). This antibody is labeled with APC-Cy7. |
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Angiostatin (ANG) Polyclonal Antibody (Mouse), APC-Cy7 |
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| 4-PAB254Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Angiostatin (ANG). This antibody is labeled with APC-Cy7. |
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Angiostatin (ANG) Polyclonal Antibody (Rat), APC-Cy7 |
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| 4-PAB254Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Angiostatin (ANG). This antibody is labeled with APC-Cy7. |
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Glucagon (GC) Polyclonal Antibody (Human), APC-Cy7 |
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| 4-PAB266Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glucagon (GC). This antibody is labeled with APC-Cy7. |
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PDGFR-induced autocrine SDF-1 signaling in most cancers cells promotes metastasis in superior pores and skin carcinoma.
Superior and undifferentiated pores and skin squamous cell carcinomas (SCCs) exhibit aggressive development and enhanced metastasis functionality, which is related in mice with an growth of the most cancers stem-like cell (CSC) inhabitants and with adjustments within the regulatory mechanisms that management the proliferation and invasion of those cells. Certainly, autocrine activation of PDGFRα induces CSC invasion and promotes distant metastasis in superior SCCs. Nevertheless, the mechanisms concerned on this course of have been unclear.
Right here, we present that CSCs of mouse superior SCCs (L-CSCs) specific CXCR4 and CXCR7, each receptors of SDF-1. PDGFRα signaling induces SDF-1 expression and secretion, and the autocrine activation of this pathway in L-CSCs.
Autocrine SDF-1/CXCR4 signaling induces L-CSC proliferation and survival, and mediates PDGFRα-induced invasion, selling in vivo lung metastasis. Validation of those findings in affected person samples of pores and skin SCCs exhibits a powerful correlation between the expression of SDF1, PDGFRA, and PDGFRB, which is upregulated, alongside CXCR4 in tumor cells of superior SCCs.
Moreover, PDGFR regulates SDF-1 expression and inhibition of SDF-1/CXCR4 and PDGFR pathways blocks distant metastasis of human PD/S-SCCs. Our outcomes point out that purposeful crosstalk between PDGFR/SDF-1 signaling regulates tumor cell invasion and metastasis in human and mouse superior SCCs, and recommend that CXCR4 and/or PDGFR inhibitors might be used to dam metastasis of those aggressive tumors.
Corrigendum Antiproliferative results of imatinib mesylate on ZR‑75‑1 and MDA‑MB‑231 cell strains by way of PDGFR‑β, PDGF‑BB, c‑Package and SCF expression.
- An reader drew to our consideration that the above research appeared to comprise a excessive stage of overlap with an article by the identical authors revealed within the journal Drug Design, Improvement and Remedy [Kadivar A, Kamalidehghan B, Akbari Javar H, Karimi B, Sedghi R and Noordin MI: Antiproliferation effect of imatinib mesylate on MCF7, T‑47D tumorigenic and MCF 10A nontumorigenic breast cell lines via PDGFR‑β, PDGF‑BB, c‑Kit and SCF genes. Drug Des Devel Ther 11: 469‑481, 2017].
- Following an inside investigation and likewise in liaison with the authors, it was established that, though the research have been performed alongside broadly comparable strains, the papers contained fully totally different knowledge involving two totally different subsets of cell strains; the submission to Drug Des Devel Ther aimed to discover the results of imatinib mesylate on three totally different teams, with every group being represented by a cell line, whereas the submission to Int J Mol Med explored the effectiveness of imatinib mesylate in breast most cancers cell strains.
- Regardless of this, contemplating the relatedness of the articles and the truth that the paper to Drug Des Devel Ther was submitted first and revealed whereas the Int J Mol Med paper was passing by the peer‑evaluation course of, the authors concede that they need to have correctly referenced their paper submitted to Drug Des Devel Ther within the Int J Mol Med paper.
- Notice that the publishers of Drug Des Devel Ther, with whom we have been liaising, agreed with the choice to concern a Corrigendum for this paper that acknowledges the article revealed in Drug Des Devel Ther.
- The authors remorse their failure to acknowledge the associated paper on this occasion, and apologize to the readership for this oversight. [the original article was published in International Journal of Molecular Medicine 14: 414‑424, 2018; DOI: 10.3892/ijmm.2018.3590].
Cy5 azide |
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| A8128-5 | ApexBio | 5 mg | EUR 741.6 |
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Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. |
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DBCO-Cy5 |
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| 923 | AAT Bioquest | 1 mg | EUR 158.4 |
CY5-SE |
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| HY-D0819 | MedChemExpress | 25mg | EUR 555.6 |
CY5-YNE |
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| HY-D0820 | MedChemExpress | 10mM/1mL | EUR 943.2 |
CY5-N3 |
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| HY-D0832 | MedChemExpress | 5mg | EUR 639.6 |
Cy5-Ctp |
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| 25801087 | Scientific Laboratory Supplies | EACH | EUR 432.06 |
Cy5-Dctp |
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| PA55021 | Scientific Laboratory Supplies | EACH | EUR 388.74 |
Cy5-Dutp |
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| PA55022 | Scientific Laboratory Supplies | EACH | EUR 388.74 |
Cy5 tyramide |
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| 11066 | AAT Bioquest | 1 mg | EUR 109 |
Cy5 aldehyde |
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| 9011 | AAT Bioquest | 5 mg | EUR 318 |
Cy5 aldehyde |
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| 9012 | AAT Bioquest | 1 mg | EUR 203 |
Cy5 tetrazine |
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| 902 | AAT Bioquest | 1 mg | EUR 222 |
Mouse anti-Human CD3, PE-Cy5 Conjugated mAb |
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| 28006-50Tests | SAB | 50 Tests | EUR 303.6 |
Mouse anti-Human CD4, PE-Cy5 Conjugated mAb |
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| 28013-50Tests | SAB | 50 Tests | EUR 303.6 |
Mouse anti-Human CD8, PE-Cy5 Conjugated mAb |
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| 28028-50Tests | SAB | 50 Tests | EUR 303.6 |
Mouse anti-Human CD14, PE-Cy5 Conjugated mAb |
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| 28053-50Tests | SAB | 50 Tests | EUR 302.4 |
Mouse anti-Human CD19, PE-Cy5 Conjugated mAb |
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| 28074-50Tests | SAB | 50 Tests | EUR 302.4 |
Mouse anti-Human CD45, PE-Cy5 Conjugated mAb |
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| 28144-50Tests | SAB | 50 Tests | EUR 303.6 |
CD117 antibody (CY5) |
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| 61R-CD117bMSCY5 | Fitzgerald | 100 ug | EUR 495.6 |
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Description: Rat monoclonal CD117 antibody (CY5) |
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CD11b antibody (CY5) |
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| 61R-CD11baMSCY5 | Fitzgerald | 100 ug | EUR 495.6 |
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Description: Rat monoclonal CD11b antibody (CY5) |
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CD19 antibody (CY5) |
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| 61R-CD19aMSCY5 | Fitzgerald | 100 ug | EUR 522 |
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Description: Rat monoclonal CD19 antibody (CY5) |
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CD19 antibody (CY5) |
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| 61R-CD19cHUCY5 | Fitzgerald | 100 tests | EUR 522 |
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Description: Mouse monoclonal CD19 antibody (CY5) |
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CD3e antibody (CY5) |
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| 61R-CD3egMSCY | Fitzgerald | 100 ug | EUR 340.8 |
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Description: Hamster monoclonal CD3e antibody (CY5) |
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CD3e antibody (CY5) |
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| 61R-CD3ehMSCY | Fitzgerald | 100 ug | EUR 340.8 |
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Description: Rat monoclonal CD3e antibody (CY5) |
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CD3 antibody (CY5) |
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| 61R-CD3gHUCY5 | Fitzgerald | 100 tests | EUR 535.2 |
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Description: Mouse monoclonal CD3 antibody (CY5) |
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CD45.1 antibody (CY5) |
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| 61R-CD45gMSCY5 | Fitzgerald | 100 ug | EUR 548.4 |
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Description: Mouse monoclonal CD45.1 antibody (CY5) |
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CD45.2 antibody (CY5) |
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| 61R-CD45hMSCY5 | Fitzgerald | 100 ug | EUR 457.2 |
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Description: Mouse monoclonal CD45.2 antibody (CY5) |
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CD45R antibody (CY5) |
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| 61R-CD45RcMSCY5 | Fitzgerald | 100 ug | EUR 495.6 |
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Description: Rat monoclonal CD45R antibody (CY5) |
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CD4 antibody (CY5) |
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| 61R-CD4eMSCY | Fitzgerald | 100 ug | EUR 483.6 |
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Description: Rat monoclonal CD4 antibody (CY5) |
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CD4 antibody (CY5) |
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| 61R-CD4kHUCY5 | Fitzgerald | 100 tests | EUR 522 |
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Description: Mouse monoclonal CD4 antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8abMSCY5 | Fitzgerald | 100 ug | EUR 495.6 |
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Description: Rat monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8adCKCY5 | Fitzgerald | 100 ug | EUR 651.6 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8aeCKCY5 | Fitzgerald | 100 ug | EUR 548.4 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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Azido-Cy5 tyramide |
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| 11061 | AAT Bioquest | 1 mg | EUR 367.2 |
PE-Cy5 Tandem |
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| 2610 | AAT Bioquest | 1 mg | EUR 262.8 |
Cy5-PEG-SH |
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| abx085228-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 1045.2 |
Cy5-PEG-SH |
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| abx085228-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 1095.6 |
Cy5-PEG-SH |
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| abx085228-34kDa50mg | Abbexa | 3.4 kDa; 50 mg | EUR 1128 |
Cy5-PEG-SH |
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| abx085228-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 1128 |
Cy5-PEG-COOH |
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| abx085357-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 598.8 |
Cy5-PEG-COOH |
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| abx085357-20kDa50mg | Abbexa | 20 kDa; 50 mg | EUR 598.8 |
Cy5-PEG-COOH |
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| abx085357-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 642 |
Cy5-PEG-COOH |
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| abx085357-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 642 |
Cy5-PEG-Mal |
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| abx085358-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 655.2 |
Cy5-PEG-Mal |
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| abx085358-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 655.2 |
Cy5-PEG-Mal |
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| abx085358-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 684 |
Cy5 NHS ester |
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| A8108-25 | ApexBio | 25 mg | EUR 895.2 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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Cy5 NHS ester |
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| A8108-5 | ApexBio | 5 mg | EUR 351.6 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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Cy5 carboxylic acid |
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| A8137-25 | ApexBio | 25 mg | EUR 1243.2 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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Cy5 carboxylic acid |
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| A8137-5 | ApexBio | 5 mg | EUR 630 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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Glucagon-Cy5 Peptide |
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| abx267068-1mg | Abbexa | 1 mg | EUR 2481.6 |
Cy5 Streptavidin 1mg |
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| PA45001 | Scientific Laboratory Supplies | EACH | EUR 247.38 |
Cy5 biotin conjugate |
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| 3100 | AAT Bioquest | 5 mg | EUR 222 |
FMOC-Lys(Cy5)-OH |
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| 5055 | AAT Bioquest | 100 mg | EUR 784.8 |
Annexin V-Cy5 Reagent |
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| 1013-1000 | Biovision | EUR 1358.4 | |
Annexin V-Cy5 Reagent |
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| 1013-200 | Biovision | EUR 483.6 | |
Cy5®-streptavidin conjugate |
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| 16913 | AAT Bioquest | 1 mg | EUR 211.2 |
Annexin V-Cy5 conjugate |
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| 20066 | AAT Bioquest | 100 Tests | EUR 262.8 |
CD45RO Antibody (PE-Cy5) |
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| abx134008-100Assays | Abbexa | 100 Assays | EUR 594 |
CD45RO Antibody (PE-Cy5) |
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| abx134008-50Assays | Abbexa | 50 Assays | EUR 477.6 |
Cy5 NHS ester(Et) |
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| A8769-25 | ApexBio | 25 mg | EUR 630 |
Cy5 NHS ester(Et) |
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| A8769-5 | ApexBio | 5 mg | EUR 351.6 |
Cy5 Bis NHS ester |
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| A8773-25 | ApexBio | 25 mg | EUR 630 |
Cy5 Bis NHS ester |
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| A8773-5 | ApexBio | 5 mg | EUR 351.6 |
Cy5 Bis carboxylic acid |
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| A8775-25 | ApexBio | 25 mg | EUR 630 |
Cy5 Bis carboxylic acid |
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| A8775-5 | ApexBio | 5 mg | EUR 351.6 |
Cy5 carboxylic acid(Et) |
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| A8777-25 | ApexBio | 25 mg | EUR 630 |
Cy5 carboxylic acid(Et) |
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| A8777-5 | ApexBio | 5 mg | EUR 351.6 |
Cy5 azide (non-sulfonated) |
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| A8113-25 | ApexBio | 25 mg | EUR 1939.2 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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Cy5 azide (non-sulfonated) |
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| A8113-5 | ApexBio | 5 mg | EUR 699.6 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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Cy5 alkyne (non-sulfonated) |
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| A8131-25 | ApexBio | 25 mg | EUR 1939.2 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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Cy5 alkyne (non-sulfonated) |
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| A8131-5 | ApexBio | 5 mg | EUR 699.6 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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Cy5 maleimide (non-sulfonated) |
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| A8139-25 | ApexBio | 25 mg | EUR 630 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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Cy5 maleimide (non-sulfonated) |
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| A8139-5 | ApexBio | 5 mg | EUR 351.6 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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Cy5 amine (non-sulfonated) |
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| A8143-25 | ApexBio | 25 mg | EUR 630 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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Cy5 amine (non-sulfonated) |
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| A8143-5 | ApexBio | 5 mg | EUR 351.6 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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Cy5 hydrazide (non-sulfonated) |
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| A8145-25 | ApexBio | 25 mg | EUR 630 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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Cy5 hydrazide (non-sulfonated) |
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| A8145-5 | ApexBio | 5 mg | EUR 351.6 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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PE-Cy5 Calibration Kit |
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| ECFP-F4-5K | Spherotech | 5X1 mL | EUR 372 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Cy5-PEG-SH,10K |
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| FL078003-10K-50mg | Biochempeg | 50mg | EUR 652.8 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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Cy5-PEG-SH,1K |
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| FL078003-1K-50mg | Biochempeg | 50mg | EUR 726 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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Cy5-PEG-SH,2K |
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| FL078003-2K-50mg | Biochempeg | 50mg | EUR 681.6 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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Cy5-PEG-SH,3.4K |
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| FL078003-3.4K-50mg | Biochempeg | 50mg | EUR 652.8 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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