Roseotoxin B alleviates cholestatic liver fibrosis through inhibiting PDGF-B/PDGFR-β pathway in hepatic stellate cells

 

Identifying effective anti-fibrotic therapies is a major clinical need that remains unmet. In the present study, roseotoxin B was shown to possess an improving effect on cholestatic liver fibrosis in bile duct-ligated mice, as proved by histochemical and immunohistochemical staining, hepatic biochemical parameters, and TUNEL apoptotic cell detection in tissue sections.

Using cellular thermal shift assay, computational molecular docking, microscale thermophoresis technology, and surface plasmon resonance biosensor, we confirmed that PDGFR-β was a direct target of roseotoxin B in fibrotic livers.

Platelet Derived Growth Factor C (PDGF-C) Antibody

Of note, human tissue microarrays detected pathologically high expression of p-PDGFR-β in liver samples of ~80% of patients with liver fibrosis and cirrhosis.
PDGF-B/PDGFR-β pathway promotes transdifferentiation and excessive proliferation of hepatic stellate cells (HSCs), which is a very crucial driver for liver fibrosis.
Meaningfully, roseotoxin B blocked the formation of PDGF-BB/PDGFR-ββ complex by targeting the D2 domain of PDGFR-β, thereby inhibiting the PDGF-B/PDGFR-β pathway in HSCs. In summary, our study provided roseotoxin B as a unique candidate agent for the treatment of liver fibrosis.

 

Activation PDGFR-α/AKT Mediated Signaling Pathways in Oral Squamous Cell Carcinoma by Mesenchymal Stem/Stromal Cells Promotes Anti-apoptosis and Decreased Sensitivity to Cisplatin.

 

 

Desmoplasia, a hallmark of a head and neck cancer, has both biologic and physiologic effects on cancer progression and chemotherapeutic response. Mesenchymal stem/stromal cells (MSCs), also known as mesenchymal stromal progenitor cells, have been shown to play a role in cancer progression, alter apoptotic responses, and confer resistance to chemotherapy in various carcinomas.

The pathophysiology of MSCs with respect to tumorigenesis is widely reported in other cancers and is sparsely reported in oral squamous cell carcinomas (OSCCs). We previously reported paracrine mediated PDGF-AA/PDGFR-α signaling to underlie MSCs chemotaxis in OSCC.

Mouse Anti-Hepatitis B Virus Core Antibody (HBcAb) ELISA Kit

Given the poor clinical response to primary chemotherapy, we hypothesized that MSCs may alter cancer cell sensitivity to cisplatin through activation of PDGFR-α mediated signaling pathways.
Co-culture of MSCs with human derived OSCC cell lines, JHU-012 and -019, resulted in a significant increase in the production of PDGF-AA and MCP-1 compared to cancer cells grown alone (p < 0.005) and was accompanied by an increase in the phosphorylation state of PDGFR-α (p < 0.02) and downstream target AKT at S473 (p < 0.025) and T308 (p < 0.02). JHU-012 and -019 cancer cells grown in co-culture were significantly less apoptotic (p < 0.001), expressed significantly higher levels of Bcl-2 (p < 0.04) with a concomitant significant decrease in bid expression (p < 0.001) compared to cancer cells grown alone.
There was a significant increase in the cisplatin dose response curve in cancer cell clones derived from JHU-012 and 019 cancer cells grown in co-culture with MSCs compared to clones derived from cancer cells grown alone (p < 0.001).
vegf-a
vegf-a
Moreover clones derived from JHU-012 cells grown in co-culture with MSCs were significantly more susceptible to cisplatin following pretreatment with, crenolanib, a PDGFR inhibitor, compared to cancer cells grown alone or in co-culture with MSCs (p < 0.0001).
These findings suggest that crosstalk between cancer cells and MSCs is mediated, at least in part, by activation of autocrine PDGF-AA/PDGFR-α loop driving AKT-mediated signaling pathways, resulting in reduced cancer cell sensitivity to cisplatin through alterations in apoptosis.

 

Mouse Monoclonal anti-human PDGF-B

hAP-0014 100ug
EUR 250

Mouse Monoclonal anti-human PDGF-B

hAP-0014A 100ug
EUR 250

PDGF-B

E8ER1914-92 100ul
EUR 275
Description: Available in various conjugation types.

PDGF-B Antibody

7199-002mg 0.02 mg
EUR 206.18
Description: PDGF-B Antibody: The platelet-derived growth factor beta (PDGF-B) is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB). Mutations in this gene are associated with meningioma , and reciprocal translocations between chromosomes 22 and 7, at sites where the PDGF-B gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans.

PDGF-B Antibody

7199-01mg 0.1 mg
EUR 523.7
Description: PDGF-B Antibody: The platelet-derived growth factor beta (PDGF-B) is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB). Mutations in this gene are associated with meningioma , and reciprocal translocations between chromosomes 22 and 7, at sites where the PDGF-B gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans.

PDGF-B Antibody

R31724 100 ug
EUR 356.15
Description: Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. PDGF-B can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGF-B plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGF-A.

PDGF-B Peptide

7199P 0.05 mg
EUR 197.7
Description: (CT) PDGF-B Peptide

Recombinant Human PDGF-B Protein, No Tag

E40KMH1525 20ug
EUR 495

PDGF-B Polyclonal Antibody

E20-73631 100ug
EUR 225
Description: Available in various conjugation types.

Polyclonal PDGF-B Antibody

APR08989G 0.1 mg
EUR 790.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PDGF-B . This antibody is tested and proven to work in the following applications:

PDGF-B Polyclonal Antibody

ABP55531-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of PDGF-B from Human, Mouse, Rat. This PDGF-B antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGF-B at AA range: 30-110

PDGF-B Polyclonal Antibody

ABP55531-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of PDGF-B from Human, Mouse, Rat. This PDGF-B antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGF-B at AA range: 30-110

PDGF-B Polyclonal Antibody

ABP55531-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of PDGF-B from Human, Mouse, Rat. This PDGF-B antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGF-B at AA range: 30-110

PDGF-B Polyclonal Antibody

ABP55531-100uL 100 μL
EUR 239
Description: Rabbit Anti-PDGF-B Polyclonal Antibody

PDGF-B Polyclonal Antibody

ABP55531-200uL 200 μL
EUR 379
Description: Rabbit Anti-PDGF-B Polyclonal Antibody

PDGF-B Polyclonal Antibody

ABP55531-30uL 30 μL
EUR 109
Description: Rabbit Anti-PDGF-B Polyclonal Antibody

PDGF-B Polyclonal Antibody

E44H02992 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Human Platelet Derived Growth Factor B (PDGF-B) Protein

abx682008-100g 100 µg
EUR 850

Human Platelet Derived Growth Factor B (PDGF-B) Protein

abx682008-20g 20 µg
EUR 325

PDGF, CF Human

PR15024CF 5 ug
EUR 1743.6

PDGF - A Human

PR27059 5 ug
EUR 229.2

PDGF - AA Human

PR27061 2 ug
EUR 229.2

PDGF - AB Human

PR27062 2 ug
EUR 229.2

PDGF - A Human

PR27059-B each
EUR 184.5

PDGF - AA Human

PR27061-B each
EUR 184.5

PDGF - AB Human

PR27062-B each
EUR 184.5

Anti-Human PDGF-A Antibody

102-P77 100 µg
EUR 245.7
Description: Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the α-granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL2 secretion accompanied by a downregulation of IL4 and IFNγ production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Anti-Human PDGF-A Antibody

101-M44 100 µg
EUR 399
Description: Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the α-granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL2 secretion accompanied by a downregulation of IL4 and IFNγ production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Anti-Human PDGF-A Antibody

101-M821 100 µg
EUR 399
Description: Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the α-granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL2 secretion accompanied by a downregulation of IL4 and IFNγ production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Anti-Human PDGF-C Antibody

101-M823 100 µg
EUR 399
Description: Mouse platelet-derived growth factor C ( PDGF-C ) is one of the four member of the PDGF family of growth factors which are known mitogens and survival factors for cells of mesenchymal origin. They form four disulfide-linked homodimers ( PDGF-AA, -BB, -CC, and –DD ) and one heterodimer ( PDGF-AB ). PDGF-C has a unique two-domain structure consisting of an N-terminal CUB and a conserved C-terminal growth factor domain that are separated by a hinge region. PDGF-CC only binds to PDGF Rα with high affinity, activates the homodimerization of PDGF Rαβ, and induces the signal pathways, such as profibrotic pathways in hepatic fibrosis. PDGF-CC is involved in a variety of processes including angiogensis, morphogenesis and cardiovascular development, as well as the tumorigenesis. Meanwhile, recent studies have shown that the serine protease tissue plasminogen activator (tPA) used for treatment of acute stroke mediates the activation PDGF-CC, which suggests potential new strategies for cardiovascular and stroke treatment.

Anti-Human PDGF-BB Antibody

102-P78 100 µg
EUR 245.7
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

PDGF B Rabbit mAb

E2R25303 100ul
EUR 255
Description: Available in various conjugation types.

PDGF B Rabbit mAb

52493 100ul
EUR 499

PDGF B Rabbit mAb

57137 100ul
EUR 339

PDGF-B Rabbit pAb

E2347269 100ul
EUR 225
Description: Available in various conjugation types.

PDGF-BB, Human

HY-P7055 10ug
EUR 321.6

PDGF-BB, human

RC216-21 2ug
EUR 125.26

Human PDGF receptor

E01A7143 96T
EUR 700
Description: ELISA

PDGF-B (L48) polyclonal antibody

E43S1290 100ul
EUR 225
Description: Available in various conjugation types.

PDGF B Rabbit Monoclonal Antibody

E10G21817 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

PDGF-B Rabbit Polyclonal Antibody

ES6530-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against PDGF-B from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

PDGF-B Rabbit Polyclonal Antibody

ES6530-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against PDGF-B from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Rabbit Polyclonal PDGF-B Antibody

TA319694 100 µg Ask for price

Recombinant Human PDGF-B Protein, Untagged, E.coli-2ug

QP10398-ec-2ug 2ug
EUR 184.8

Recombinant Human PDGF-B Protein, Untagged, E.coli-1mg

QP5307-1mg 1mg
EUR 4386

Human Platelet-Derived Growth Factor-B,PDGF-B ELISA kit

201-12-2119 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Platelet-Derived Growth Factor-B(PDGF-B)ELISA Kit

QY-E00606 96T
EUR 480

Human Platelet-Derived Growth Factor-B,PDGF-B ELISA kit

YLA1163HU-48T 48T Ask for price

Human Platelet-Derived Growth Factor-B,PDGF-B ELISA kit

YLA1163HU-96T 96T Ask for price

Recombinant Human PDGF-B Protein, Untagged, E.coli-10ug

QP10398-ec-10ug 10ug
EUR 348

Recombinant Human PDGF-B Protein, Untagged, E.coli-100ug

QP10398-ec-100ug 100ug
EUR 1522.8

Recombinant Human PDGF-B Protein, Untagged, E.coli-250ug

QP10398-ec-250ug 250ug
EUR 2686.8

Recombinant Human PDGF-B Protein, Untagged, E.coli-500ug

QP5318-500ug 500ug
EUR 2960.4

PDGF-B Recombinant Protein

96-936 0.05 mg
EUR 651.3
Description: PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.

Human Platelet-Derived Growth Factor-B, PDGF-B GENLISA ELISA

KBH2118 1 x 96 wells
EUR 286

Human PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit

EKE61006-5x96T 5x96T
EUR 3124.08

Human PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit

EKE61006-96T 96T
EUR 657.7

Human PDGF-B(Platelet Derived Growth Factor Subunit B) ELISA Kit

RE3074H-48wells 48 wells
EUR 125.55

Human PDGF-B(Platelet Derived Growth Factor Subunit B) ELISA Kit

RE3074H-96wells 96 wells
EUR 179.55

Genorise® Human PDGF-B Expression Plasmid, 10 ug

GR163018 10 µg
EUR 219

PDGF-B Platelet-Derived Growth Factor B Human Recombinant Protein

PROTP01127-1 Regular: 20ug
EUR 380.4
Description: PDGF-B Human Recombinant mature chain produced in E.Coli is a non-glycosylated, polypeptide chain containing 109 amino acids fragment (82-190) and having a molecular mass of 16.75 kDa. The PDGF-B is fused with an amino-terminal hexahistidine tag and purified by proprietary chromatographic techniques.

Human PDGF ELISA Kit

EHP0040 96Tests
EUR 625.2

rec PDGF BB (human)

4031083.0002 2 µg
EUR 102.27

rec PDGF BB (human)

4031083.001 10 µg
EUR 271.85

rec PDGF BB (human)

H-7530.0002 2.0µg
EUR 194.4

rec PDGF BB (human)

H-7530.0010 10.0µg
EUR 457.2

rec PDGF AA (human)

H-7640.0002 2.0µg
EUR 194.4

rec PDGF AA (human)

H-7640.0010 10.0µg
EUR 457.2

Human PDGF-BB Protein

abx060208-100ug 100 ug
EUR 627.6

Human PDGF-BB Protein

abx060258-100ug 100 ug
EUR 627.6

Human PDGF-BB Protein

abx060208-100g 100 µg Ask for price

Human PDGF-BB Protein

abx060208-10g 10 µg
EUR 243.75

Human PDGF-BB Protein

abx060208-50g 50 µg
EUR 437.5

PDGF ELISA KIT|Human

EF007049 96 Tests
EUR 826.8

Human Platelet Derived Growth Factor Subunit B(PDGF-B) Elisa Kit

EK710739 96 Wells
EUR 0.11

Human Platelet Derived Growth Factor Subunit B (PDGF-B)ELISA Kit

SL2524Hu 96 Tests
EUR 468

Human PDGF-αR ELISA Kit

EHP0039 96Tests
EUR 625.2

Genorise® Goat Anti-porcine PDGF-B polyclonal antibody

GR105219 100 ug
EUR 438

Mouse Monoclonal anti-human PDGF-C

hAP-0567 100ug
EUR 250

Recombinant Human PDGF-BB

EPT197 10ug
EUR 167.4
Description: Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.

Recombinant Human PDGF-AA

HEGFP-0508 10ug Ask for price

Recombinant Human PDGF-BB

HEGFP-1601 10ug Ask for price

Recombinant Human PDGF-BB

SJA04-01 10µg/vial
EUR 307.2

Mouse Monoclonal anti-human PDGF-AA

hAP-0013 100ug
EUR 250

The emerging complexity of PDGFRs: activation, internalization and signal attenuation.

The platelet-derived growth factor receptor (PDGFR) family of receptor tyrosine kinases allows cells to communicate with the environment to regulate diverse cellular activities.
Here, we highlight recent data investigating the structural makeup of individual PDGFRs upon activation, revealing the importance of the whole receptor in the propagation of extracellular ligand binding and dimerization.
Furthermore, we review ongoing research demonstrating the significance of receptor internalization and signal attenuation in the regulation of PDGFR activity.

Human Anti-Brucella IgM ELISA Kit

Interactions with internalization machinery, signaling from endosomes, receptor degradation and receptor recycling are physiological means by which cells fine-tune PDGFR responses to growth factor stimulation.
In this review, we discuss the biophysical, structural, in silico and biochemical data that have provided evidence for these mechanisms. We further highlight the commonalities and differences between PDGFRα and PDGFRβ signaling, revealing critical gaps in knowledge.
In total, this review provides a conclusive summary on the state of the PDGFR field and underscores the need for novel techniques to fully elucidate the mechanisms of PDGFR activation, internalization and signal attenuation.

 

Human PDGF-BB Protein

abx060258-100ug 100 ug
EUR 627.6

Human PDGF-BB Protein

abx060208-100g 100 µg Ask for price

Human PDGF-BB Protein

abx060208-10g 10 µg
EUR 243.75

Human PDGF-BB Protein

abx060208-50g 50 µg
EUR 437.5

Recombinant Human PDGF-BB

HEGFP-1601 10ug Ask for price

Recombinant Human PDGF-BB

EPT197 10ug
EUR 167.4
Description: Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.

Recombinant Human PDGF-BB

SJA04-01 10µg/vial
EUR 307.2

PDGF-BB, human recombinant

4488-10 each
EUR 398.4

PDGF-BB, human recombinant

4488-1000 each
EUR 8346

PDGF-BB, human recombinant

4488-50 each
EUR 1123.2

PDGF-BB/ Rat PDGF- BB ELISA Kit

ELA-E0633r 96 Tests
EUR 1063.2

PDGF BB

LF-PR012 10 ug
EUR 280.8
Description: PDGF BB protein

PDGF-BB

90800-1 10 µg
EUR 215
Description: Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in an E. coli expression system.

PDGF-BB

90800-2 100 µg
EUR 1185
Description: Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in an E. coli expression system.

PDGF-BB

90800-3 1000 µg
EUR 4320
Description: Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in an E. coli expression system.

PDGF-BB

E21-199 10ug
EUR 225
Description: Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.

PDGF-BB

PR15027 50 ug
EUR 475.2

Human PDGF-BB ELISA Kit

E16HE0073 96T
EUR 833.33

Human PDGF-BB ELISA Kit

EHP0050 96Tests
EUR 625.2

Human PDGF-BB ELISA Kit

EHP0634 96Tests
EUR 625.2

Human PDGF-BB ELISA Kit

GWB-SKR056 96 Tests Ask for price

Human PDGF-BB ELISA Kit

RK00323 96T
EUR 280

Human PDGF-BB ELISA Kit

RK04112 96 Tests
EUR 280

PDGF BB antibody

70R-PR020 50 ug
EUR 312
Description: Affinity purified Rabbit polyclonal PDGF BB antibody

PDGF BB Antibody

GWB-8EA460 0.025 mg Ask for price

Sheep Polyclonal anti-human PDGF-BB

hAP-5392 50ug
EUR 400

PDGF-BB Antibody

5488-200 each
EUR 379.2

PDGF-BB Antibody

5488-30T each
EUR 175.2

PDGF-BB Antibody

5489-100 each
EUR 379.2

PDGF-BB Antibody

5489-30T each
EUR 175.2

Human PDGF-BB GENLISA ELISA

KB1100 1 x 96 wells
EUR 297

PDGF-BB ELISA KIT|Human

EF000205 96 Tests
EUR 826.8

PDGF-BB (Human) ELISA Kit

EKA51872-5x96T 5x96T Ask for price

PDGF-BB (Human) ELISA Kit

EKA51872-96T 96T
EUR 446.9

Human PDGF-BB Recombinant Protein

200-055 5 µg
EUR 52.5
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Human PDGF-BB Recombinant Protein

200-055S 2 µg
EUR 47.25
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Human PDGF-BB Recombinant Protein

200-056 20 µg
EUR 136.5
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Recombinant Human PDGF-BB Protein

E40KMP1742 20ug
EUR 495

Recombinant Human PDGF-BB Protein

PROTP01127-4 10ug
EUR 380.4
Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Recombinant Human PDGF-BB Protein

RP00834 10 μg
EUR 265.2

Recombinant Human PDGF-BB Protein

RP01763 100μg
EUR 195

PDGF-BB, human recombinant protein

P1047-.01 10 µg
EUR 222
Description: PDGF-BB, platelet-derived growth factor, expressed in E.Coli.

PDGF-BB, human recombinant protein

P1047-.05 50 µg
EUR 692
Description: PDGF-BB, platelet-derived growth factor, expressed in E.Coli.

PDGF-BB, human recombinant protein

P1047-1 1 mg
EUR 5414
Description: PDGF-BB, platelet-derived growth factor, expressed in E.Coli.

PDGF-BB (P.pastoris-expressed), Human

HY-P7055A 10ug
EUR 321.6

PDGF-BB, CF

PR15026CF 50 ug
EUR 1304.4

PDGF-BB, CF

PR15027CF 50 ug
EUR 475.2

rHu PDGF-BB

AK8292-0002 2µg Ask for price

rHu PDGF-BB

AK8292-0010 10µg Ask for price

rHu PDGF-BB

AK8292-0100 100µg Ask for price

rHu PDGF-BB

AK8292-1000 1mg Ask for price

PDGF-BB, Rat

HY-P7278 50ug
EUR 459.6

Nori Human PDGF-BB ELISA Kit

GR111035 96-well
EUR 461

PDGF BB protein

30R-AP004 2 ug
EUR 312
Description: Purified recombinant Human PDGF BB protein

PDGF BB protein

30R-AP051 10 ug
EUR 312
Description: Purified recombinant Human PDGF BB protein

PDGF BB protein

30R-2611 10 ug
EUR 364
Description: Purified recombinant Human PDGF BB protein

PDGF BB protein

30R-2612 10 ug
EUR 280
Description: Purified recombinant Mouse PDGF BB protein

PDGF-BB, Mouse

HY-P7087 100ug
EUR 1135.2

Human/Rat PDGF-BB ELISA Kit

E28L0369 96T
EUR 666.67

Anti-Human PDGF-BB Rabbit Polyclonal Antibody

TA328417 100 µg Ask for price

Human PDGF-BB PicoKine ELISA Kit

EK0956 96 wells
EUR 510
Description: For quantitative detection of human PDGF-BB in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Human PDGF-BB Protein, premium grade

PDB-H4112 20ug
EUR 2354
Description: Human PDGF-BB, premium grade (PDB-H4112) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).

Genorise® Recombinant Human PDGF-BB

GR104258 5 µg
EUR 285

PDGF BB Homodimer Antibody

GWB-8B95AD 0.1 mg Ask for price

PDGF BB Homodimer Antibody

GWB-9A340A 0.1 mg Ask for price

Recombinant Human PDGF-BB Protein (Active)

PKSH032906-10ug 10ug
EUR 178
Description: Human

Recombinant Human PDGF-BB Protein (Active)

PKSH032906-50ug 50ug
EUR 523
Description: Human

Polyclonal PDGF-BB Antibody

APR00412G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PDGF-BB . This antibody is tested and proven to work in the following applications:

Polyclonal PDGF-BB Antibody

APR08990G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PDGF-BB . This antibody is tested and proven to work in the following applications:

Human PDGF-BB Recombinant Protein Lyophilized

IHUPDGFBBRLY10UG each
EUR 535
Description: Human PDGF-BB Recombinant Protein Lyophilized

Human PDGF-BB Recombinant Protein Lyophilized

IHUPDGFBBRLY2UG each
EUR 341
Description: Human PDGF-BB Recombinant Protein Lyophilized

Human CellExp™ PDGF-BB, human recombinant

7276-10 each Ask for price

Human PDGF-BB ELISA kit ELISA kit

E22-HC181.48 48T
EUR 295

Human PDGF-BB ELISA kit ELISA kit

E22-HC181.96 96T
EUR 395

Recombinant Human Platelet-Derived Growth Factor BB/PDGF-BB

AP74931 1mg
EUR 3209

Recombinant Human Platelet-Derived Growth Factor BB/PDGF-BB

C199-10ug 10ug
EUR 242.4
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Recombinant Human Platelet-Derived Growth Factor BB/PDGF-BB

C199-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Recombinant Human Platelet-Derived Growth Factor BB/PDGF-BB

C199-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Recombinant Human Platelet-Derived Growth Factor BB/PDGF-BB

C199-50ug 50ug
EUR 595.2
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Human PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit

EKE60948-5x96T 5x96T
EUR 3124.08

Human PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit

EKE60948-96T 96T
EUR 657.7

Human PDGF-BB(Platelet Derived Growth Factor BB) ELISA Kit

RE3064H-48wells 48 wells
EUR 125.55

Human PDGF-BB(Platelet Derived Growth Factor BB) ELISA Kit

RE3064H-96wells 96 wells
EUR 179.55

PDGF-BB (His tag)

EGPS006 10ug
EUR 150

Human PDGF-BB -Platelet Derived Growth Factor-BB- CLIA Kit

E-CL-H1012-24Tests 24 Tests
EUR 180
Description: Sandwich

Human PDGF-BB -Platelet Derived Growth Factor-BB- CLIA Kit

E-CL-H1012-48Tests 48 Tests
EUR 546
Description: Sandwich

Human PDGF-BB -Platelet Derived Growth Factor-BB- CLIA Kit

E-CL-H1012-96Tests 96 Tests
EUR 682
Description: Sandwich

Human PDGF-BB -Platelet Derived Growth Factor-BB- CLIA Kit

E-CL-H1012-96Tests10 96 Tests *10
EUR 6820
Description: Sandwich

Human PDGF-BB -Platelet Derived Growth Factor-BB- CLIA Kit

E-CL-H1012-96Tests5 96 Tests *5
EUR 3410
Description: Sandwich

Human PDGF-BB(Platelet Derived Growth Factor-BB) ELISA Kit

EH0254 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml